Package: Bowtie2¶

About: Short-read alignment program.

Version: 2.2.9

Added: 2016-04-21

Updated: 2016-04-21

Installation directory: /shares/bioinfo/bin

Source directory: /shares/bioinfo/installs/bowtie2-2.2.9

Link: Bowtie2

bowtie2¶

Example bowtie2 HTCondor job file¶

• requests resources (CPUs, RAM, scratch)
• transfers pair1 and pair2 into scratch
• all output file(s) are automatically transferred to working directory at end of job

universe                 = vanilla
getenv                   = true

accounting_group         = $ENV(CONDOR_GROUP)
condor_output            = /path/to/condor/output/dir/

request_cpus             = $(cpu_req)
request_memory           = $(memory_req)
request_disk             = $(disk_req)

notification             = Complete

ID                       = $(Cluster).$(Process)
output                   = $(condor_output)$(run_type).$(ID).out
error                    = $(condor_output)$(run_type).$(ID).err
log                      = $(condor_output)$(run_type).$(ID).log
###################

executable               = /shares/bioinfo/bin/bowtie2
arguments                = -q --threads $(cpu_req) -x /path/to/genome.fa -1 pair1.reads.fastq.qz -2 pair2.reads.fastq.gz -S alignment.sam


transfer_executable      = False
should_transfer_files    = YES
when_to_transfer_output  = ON_EXIT
transfer_input_files     = [full/path/to/pair1.reads.fastq.gz,full/path/to/pair2.reads.fastq.gz]


## Need to edit info here ##
run_type                 = bowtie2
cpu_req                  = [Number of processors]
memory_req               = [RAM request i.e. 1G]
disk_req                 = [scratch disk size request 3G]
############################

queue